Abcam, ab113103, Anti-TRMT12 antibody

Size: 100µg
Rabbit Polyclonal TRMT12 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TRMT12.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TRMT12. The exact immunogen used to generate this antibody is proprietary information.Q53H54,
Specificity:ab113103 is predicted to not cross-react with other TRMT protein family members. At least two isoforms of TRMT12 are known to exist, ab113013 will detect both isoforms.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab113103 is purified by affinity chromatography via a peptide column., Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRMT12 also known as TRM12 or NML1 is an enzyme that catalyzes the methylation of tRNA molecules. The protein has a mass of approximately 54 kDa and is concentrated in the nucleolus of cells. TRMT12 is expressed in various tissues with high levels in the liver and brain. Its mechanical role involves adding a methyl group at a specific location on the tRNA which is critical in maintaining proper tRNA structure and function.
Biological function summary
TRMT12 contributes to the post-transcriptional modification of tRNA which is essential for accurate and efficient translation of the genetic code into proteins. It forms a part of a larger enzyme complex responsible for modifying different bases in tRNA. This modification allows the tRNA to correctly recognize and pair with mRNA codons during protein synthesis ensuring accurate protein translation.
Pathways
TRMT12 plays an essential role in the tRNA modification pathway and impacts protein synthesis quality control. It associates with pathways that involve protein translation and processing ensuring cellular proteins are produced with correct amino acid sequences. It interacts with other proteins such as TRMT10A and TRMT61B which are involved in multiple steps of tRNA processing and modification.
TRMT12 has been implicated in conditions such as intellectual disability and certain forms of cancer. Deficient tRNA methylation can lead to improper protein synthesis contributing to disease states. TRMT12 is linked with proteins such as DNMT3A which are also related to methylation processes and are involved in cancer development and disease progression. Improved comprehension of how TRMT12 functions may reveal therapeutic targets for intervention in these conditions.