Abcam, ab114310, Recombinant Human 5 Lipoxygenase/5-LO protein

Size: 10µg
Recombinant Human 5 Lipoxygenase/5-LO protein is a Human Full Length protein, in the 1 to 674 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P09917,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.3% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
5-Lipoxygenase also known as 5-LO or 5-LOX is an enzyme that catalyzes the oxidation of arachidonic acid to produce leukotrienes which are signaling molecules in the body. 5-LOX has a molecular weight of approximately 78 kDa. It is mainly expressed in leukocytes like macrophages neutrophils and mast cells. This enzyme plays an important role in the inflammatory response and the regulation of immune functions in these cells.
Biological function summary
The activity of 5-Lipoxygenase contributes to the production of leukotrienes which are pivotal in mediating inflammatory and allergic responses. This enzyme operates alone but it often interacts with 5-LOX-activating protein (FLAP) to facilitate the synthesis of leukotrienes efficiently. These interactions suggest that 5-LOX has a significant role in cellular processes related to inflammation and immune reactions.
Pathways
5-Lipoxygenase is integral to the arachidonic acid metabolic pathway initiating the biosynthesis of leukotrienes from arachidonic acid. This pathway also involves other enzymes like phospholipase A2 which releases arachidonic acid from membrane phospholipids and cyclooxygenase enzymes (COX) which convert arachidonic acid into prostaglandins. The end products of this pathway including leukotrienes manage and resolve inflammation and immune responses illustrating the collaborative effort between these enzymes.
5-Lipoxygenase is significantly linked to inflammatory diseases such as asthma and atherosclerosis. In asthma the overproduction of leukotrienes due to enhanced 5-LOX activity results in bronchoconstriction and inflammation of the airways. In atherosclerosis leukotrienes contribute to the development and progression of plaques driving chronic inflammation in the vascular system. Additionally through its pathway its activity relates closely to cyclooxygenase enzymes showing its importance in disease mechanisms targeted by anti-inflammatory therapies.