Abcam, ab114591, Recombinant Human ACADL/LCAD protein

Size: 10µg
Recombinant Human ACADL/LCAD protein is a Human Full Length protein, in the 1 to 430 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P28330,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.3% Glutathione

Product details:
This product was previously labelled as ACADL.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ACADL also known as LCAD (Long-Chain Acyl-CoA Dehydrogenase) is an enzyme important for fatty acid metabolism. It has a molecular mass of approximately 43 kDa. This protein is mainly found in mitochondria and you can find high expression levels in tissues with significant energy demands like liver muscle and heart. The mechanical function involves catalyzing the initial step in the beta-oxidation cycle which is the oxidation of long-chain acyl-CoA substrates to form a trans-double bond between the beta and alpha carbons.
Biological function summary
ACADL plays an essential role in mitochondrial energy production. It is part of the electron transport chain more specifically involved in the oxidation of fatty acids. This process is central to sustaining energy and heat production especially during fasting. ACADL works as part of a multi-enzyme complex known as the mitochondrial trifunctional protein complex responsible for multiple steps in fatty acid oxidation.
Pathways
ACADL's function in beta-oxidation links it to key metabolic pathways like energy metabolism and fatty acid catabolism. It directly interacts and works with other proteins such as enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase which facilitate subsequent steps after ACADL's initial action in the same beta-oxidation pathway. This integration ensures the proper breakdown of fatty acids to acetyl-CoA feeding into the citric acid cycle for ATP production.
Defects in ACADL activity are implicate in metabolic conditions such as long-chain acyl-CoA dehydrogenase deficiency (LCADD) and related cardiomyopathies. LCADD can lead to severe symptoms involving muscle weakness cardiomyopathy and hypoglycemia. ACADL's role in these disorders often intersects with other proteins like VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) since deficiencies or dysfunctions can manifest in overlapping symptoms due to their shared role in fatty acid oxidation.