Abcam, ab11751, Anti-alpha 1 Spectrin antibody [17C7]

Size: 100µL
Anti-alpha 1 Spectrin antibody [17C7] (ab11751) is a mouse monoclonal antibody detecting alpha 1 Spectrin in Western Blot, IHC-Fr, ICC/IF, ELISA . Suitable for Human, Mouse, Rat . - Over 10 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:17C7,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-Fr, WB, ICC/IF, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human SPTA1.P02549,
Specificity:ab11751 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ab11751 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes.

Product details:
What is this antibody validated in?
Anti-alpha 1 Spectrin antibody [17C7] (ab11751) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse, Rat samples.
What is the molecular weight of alpha 1 Spectrin?
Anti-alpha 1 Spectrin [17C7] (ab11751) specifically detects a band for alpha 1 Spectrin (UniProt: P02549) at a molecular weight of 280kDa.
Trusted by the scientific community
Anti-alpha 1 Spectrin [17C7] (ab11751) was first used in a scientific publication in 2004 and has been cited over 10 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from tissue culture supernatant., Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha 1 Spectrin also known as SPTA1 is an essential protein that plays a mechanical role in cells. This protein has a large molecular mass of approximately 280 kDa and is predominantly expressed in erythrocytes but it is also found in other cell types. Structurally it forms part of the spectrin family which includes alpha and beta spectrin subunits. Alpha 1 Spectrin interacts with the actin cytoskeleton creating a network that supports the plasma membrane’s integrity and flexibility.
Biological function summary
Alpha 1 Spectrin binds with other proteins to form a spectrin-actin complex that is essential for maintaining cell shape and mechanical stability. It interacts with ankyrin and other components of the cytoskeleton to provide the necessary elasticity and durability to cells especially red blood cells. This protein makes sure that the cytoskeletal network remains stable and efficiently responds to mechanical stresses from the outside environment.
Pathways
Alpha 1 Spectrin is involved in critical pathways important for cellular functions. It participates in the erythrocyte cytoskeleton organization pathway interacting with proteins such as ankyrin and protein 4.1R. These interactions influence cell deformability and help maintain the biconcave shape of red blood cells allowing them to navigate through narrow capillaries effectively. Alpha 1 Spectrin’s functionality ensures efficient oxygen delivery throughout the body by maintaining red blood cell integrity.
Mutations or deficiencies in Alpha 1 Spectrin can lead to conditions such as hereditary spherocytosis and elliptocytosis. These disorders are characterized by hemolytic anemia and are linked to disruptions in the cytoskeleton structure and function of erythrocytes. In these cases Alpha 1 Spectrin can display abnormal interactions with related proteins like ankyrin leading to compromised membrane stability and shortened red blood cell lifespan. The understanding of Alpha 1 Spectrin’s role in these diseases provides insight into potential therapeutic targets for managing these disorders.