Abcam, ab117757, Anti-Endothelin 1 antibody

Size: 100µL
Anti-Endothelin 1 antibody (ab117757) is a rabbit polyclonal antibody detecting Endothelin 1 in Western Blot, IHC-P . Suitable for Human, Mouse . - Over 20 publications
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Edn1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P22387

Product details:
What is this antibody validated in?
Anti-Endothelin 1 antibody (ab117757) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.
What is the molecular weight of Endothelin 1?
Anti-Endothelin 1 (ab117757) specifically detects a band for Endothelin 1 (UniProt: P05305) at a molecular weight of 24kDa.
Trusted by the scientific community
Anti-Endothelin 1 (ab117757) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Preservative: 0.02% Sodium azideConstituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Endothelin 1 (ET-1) is a potent vasoconstrictor peptide with a molecular mass of approximately 2.5 kDa. The gene encoding ET-1 is expressed predominantly in endothelial cells where it exerts its vasoconstrictive effect. It is synthesized as a prepropeptide that undergoes cleavage to form the mature ET-1 peptide. The primary expression occurs in the vascular endothelial cells but it is also found in the kidney lung and brain indicating its widespread physiological role.
Biological function summary
ET-1 regulates vascular tonus and blood pressure. It is part of the endothelin family that includes endothelin-2 (ET-2) and endothelin-3 (ET-3). ET-1 binds to endothelin receptors such as ETA and ETB which are G protein-coupled receptors facilitating cellular signaling processes. These receptors are abundant in smooth muscle cells and the central nervous system. The interaction of ET-1 with these receptors affects not only vascular smooth muscle contraction but also cell proliferation and production of extracellular matrix proteins.
Pathways
ET-1 plays an important role in the renin-angiotensin-aldosterone system and the nitric oxide signaling pathway. ET-1 interacts closely with angiotensin II a peptide hormone that increases blood pressure by stimulating the production of endothelin in endothelial cells. The release of nitric oxide by endothelium serves as a counter-regulatory mechanism to ET-1-mediated vasoconstriction. The balance between ET-1 and nitric oxide is vital for maintaining vascular homeostasis.
ET-1 is significantly implicated in hypertension and pulmonary arterial hypertension (PAH). The overproduction of ET-1 contributes to sustained high blood pressure and arterial stiffness. In PAH ET-1 levels are increased leading to the constriction and proliferation of pulmonary vascular cells. ET-1's interaction with proteins like transforming growth factor-beta (TGF-Β) further enhances its role in vascular remodeling associated with these conditions. Understanding ET-1's interaction in these pathways has made it a therapeutic target for treating these cardiovascular diseases.