Abcam, ab119603, Mouse LOX1 ELISA Kit (OLR1)

Size: 1 x 96Tests
Mouse LOX1 ELISA Kit (OLR1) is a Sandwich (quantitative) ELISA for the measurement of Mouse LOX1 (OLR1) in Mouse in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 1 pg/mL,
Range:31.2 - 2000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's mouse LOX1 (OLR1)
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse LOX1 in cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA)
A LOX1 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for LOX1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse LOX1 amount of sample captured in plate.
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C