Abcam, ab213798, Human IL-37 ELISA Kit

Size: 1 x 96Tests
Human IL-37 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-37 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:15.6 - 1000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human IL-37 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213798) is designed for the quantitative measurement of Human IL-37 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-37 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: V46-D218) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-37 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-37 amount of sample captured in plate.
Interleukin 37 also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family, and it is mapped to 2q13.
In vitro
expression analysis showed that IL-37 was a secretory protein. IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IL-37 also known as IL-1F7 is a member of the interleukin 1 cytokine family. This protein has a molecular mass of approximately 25 kDa. IL-37 is mainly expressed in tissues like the kidney thymus testis and uterus with lower levels in the bone marrow and placenta. Researchers identify this protein in various cells such as monocytes which are key players in the immune system.
Biological function summary
IL-37 functions within the immune response by acting as a critical anti-inflammatory cytokine. It does not form a larger complex for its activity but instead interacts directly with various immune cells. IL-37 is capable of reducing pro-inflammatory cytokine production and dampening the immune response when necessary thereby providing a mechanism to prevent excessive inflammation and tissue damage.
Pathways
IL-37 plays a role in pathways like the inflammatory response and immune regulation. Within these systems it interacts with proteins such as IL-18 and IL-18BP (binding protein) which helps modulate its activity. IL-37 can also influence the NF-kB pathway an essential pathway in regulating the immune response by indirectly reducing inflammation.
IL-37 is associated with conditions such as rheumatoid arthritis and inflammatory bowel disease. These disorders involve chronic inflammation where IL-37 might serve a protective role by reducing excessive inflammatory signals. In these contexts IL-37 interacts with other inflammatory cytokines like IL-6 and TNF-α which are known to be elevated in such diseases. By potentially mitigating these signals IL-37 helps in managing the inflammatory aspects of these disorders.