Abcam, ab213897, Mouse VEGF-B ELISA Kit

Size: 1 x 96Tests
Mouse VEGF-B ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse VEGF-B in Mouse in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Heparin Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 2 pg/mL,
Range:31.2 - 2000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Mouse VEGF-B Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213897) is designed for the quantitative detection of Mouse VEGF-B in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for VEGF-B has been precoated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: Q20-A207 and Q20-R148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF-B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse VEGF-B amount of sample captured in plate.
Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motor neurons during motor neuron diseases such as amyotrophic lateral sclerosis.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VEGFB also known as vascular endothelial growth factor B is a member of the VEGF family. It has a molecular mass of approximately 34-35 kDa. VEGFB interacts with its receptors to promote angiogenesis increasing blood supply to tissues. Expression of VEGFB occurs in the heart skeletal muscle and various endothelial cells. It also shows expression in tissues undergoing high metabolic activities. Besides its angiogenic role VEGFB exhibits participation in metabolic regulation.
Biological function summary
VEGFB supports angiogenesis by promoting endothelial cell growth and migration. It acts as a signal protein not forming part of a larger complex. VEGFB binds to VEGFR-1 (Flt-1) receptor which is involved in transmitting the growth-promoting signals. Its function extends to the regulation of fatty acid uptake into tissues highlighting its role in lipid metabolism and energy utilization.
Pathways
The signalling activities of VEGFB are significant in angiogenesis and metabolic pathways. VEGFB interacts closely with the VEGF-A pathway which is vital for forming new blood vessels. It also shares interactive roles with similar proteins such as VEGF-C and VEGF-D contributing to various angiogenic processes and cellular growth signalling systems.
Understanding VEGFB's role is important in conditions like cardiovascular disease and metabolic syndrome. Altered VEGFB signalling may link to heart diseases due to its regulatory function in maintaining heart vasculature. VEGFB has connections with VEGFR-1 and VEGF-A indicating its potential involvement in endothelial dysfunction and highlighting its significance in therapeutic strategies targeting such diseases.