Abcam, ab218607, Torin 2, mTOR inhibitor

Size: 5mg / 25mg
MW 432.4 Da, Purity >98%. Potent, selective, ATP-competitive mTOR inhibitor. Inhibits mTORC1 (IC 50 = 2.1 nM) in in vitro kinase activity assays. Displays 800-fold selectivity for mTOR over PI3K (cellular EC 50 values are 0.25 and 200 nM for mTOR and PI3K respectively). Attenuates phosphorylation of mTOR downstream targets, inhibits cell proliferation of several cancer cell types, and induces apoptosis and autophagy. Also exhibits potent biochemical and cellular activity against phosphatidylinositol-3 kinase-like kinase (PIKK) family kinases including ATM, ATR and DNA-PK (EC 50 values are 28 nM, 35 nM and 118 nM respectively). Has significantly improved bioavailability (54%), metabolic stability, and plasma exposure compared to Torin 1 (ab218606).
Key facts
CAS number:1223001-51-1,
Purity:>98%,
Form:SolidSee storage information,
Molecular weight:432.4 Da,
Molecular formula:C24H15F3N4O,
PubChem:51358113,
Nature:Synthetic,
Solubility:Soluble in DMSO to 20 mM,
Biochemical name:Torin 2,
Biological description:Potent, selective, ATP-competitive mTOR inhibitor. Inhibits mTORC1 (IC50 = 2.1 nM) in in vitro kinase activity assays. Displays 800-fold selectivity for mTOR over PI3K (cellular EC50 values are 0.25 and 200 nM for mTOR and PI3K respectively). Attenuates phosphorylation of mTOR downstream targets, inhibits cell proliferation of several cancer cell types, and induces apoptosis and autophagy. Also exhibits potent biochemical and cellular activity against phosphatidylinositol-3 kinase-like kinase (PIKK) family kinases including ATM, ATR and DNA-PK (EC50 values are 28 nM, 35 nM and 118 nM respectively). Has significantly improved bioavailability (54%), metabolic stability, and plasma exposure compared to Torin 1 (ab218606).,
Canonical smiles:C1=CC(=CC(=C1)N2C(=O)C=CC3=CN=C4C=CC(=CC4=C32)C5=CN=C(C=C5)N)C(F)(F)F,
InChi:InChI=1S/C24H15F3N4O/c25-24(26,27)17-2-1-3-18(11-17)31-22(32)9-6-16-13-29-20-7-4-14(10-19(20)23(16)31)15-5-8-21(28)30-12-15/h1-13H,(H2,28,30),
InChiKey:GUXXEUUYCAYESJ-UHFFFAOYSA-N,
IUPAC Name:9-(6-aminopyridin-3-yl)-1-[3-(trifluoromethyl)phenyl]benzo[h][1,6]naphthyridin-2-one

Product details:
This product is manufactured by BioVision, an Abcam company and was previously called 2274 Torin 2. 2274-25 is the same size as the 25 mg size of ab218607.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Store under desiccating conditions

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The mammalian target of rapamycin commonly known as mTOR is a serine/threonine kinase known for its role in cellular growth and metabolism. It has a molecular weight of approximately 289 kDa. mTOR is expressed in various tissues throughout the body including muscle adipose tissue and the brain. The protein functions as a central regulator of cell proliferation protein synthesis and nutrient signaling. Often researchers utilize mTOR ELISA or mTOR western blot (mTOR WB) methods and mTOR antibodies to study its expression and activity in various biological contexts.
Biological function summary
MTOR integrates signals from nutrients growth factors and cellular energy status to maintain cellular homeostasis. It forms part of two distinct complexes mTORC1 and mTORC2 which differ in their component proteins and downstream effects. mTORC1 primarily responds to amino acids and regulates protein synthesis through phosphorylation of key substrates like S6K1. On the other hand mTORC2 is important for maintaining cytoskeletal integrity and cell survival highlighting the protein's importance in diverse cellular processes.
Pathways
MTOR plays a pivotal role in the PI3K/AKT/mTOR pathway which governs cell growth proliferation and survival. It also has implications in the regulation of the AMPK pathway which senses cellular energy levels. Through these pathways mTOR interacts with proteins such as AKT and TSC2. The phospho-mTOR specifically the S2448 phospho-mTOR serves as an important functional marker in these signaling cascades linking extracellular signals to downstream cellular responses.
MTOR has connections to cancer and neurodegenerative diseases. Its dysregulation often leads to uncontrolled cellular proliferation a hallmark of many cancers. Conditions such as tuberous sclerosis can occur due to mutations in proteins like TSC1 and TSC2 that regulate mTOR activity. In Alzheimer's disease mTOR's role in autophagy and protein synthesis becomes significant as imbalance may contribute to disease progression. Understanding these connections highlights the potential of targeting mTOR pathways therapeutically.