Abcam, ab219049, Human Hemoglobin subunit alpha ELISA Kit

Size: 1 x 96Tests
Human Hemoglobin subunit alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Hemoglobin subunit alpha with a sensitivity of 131 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 131 pg/mL,
Range:588 - 20000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Hemoglobin subunit alpha ELISA Kit (ab219049) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemoglobin subunit alpha protein in cerebral spinal fluid, cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hemoglobin subunit alpha with 131 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Hemoglobin (Haemoglobin) subunits combine to form the quaternary structure of total hemoglobin, the oxygen carrying protein in red blood cells. In normal adults hemoglobin consists of 2 alpha and 2 beta subunits. Hemoglobin alpha subunit can be modified by the glucose on the terminal valine of beta subunits resulting in the Hemoglobin A1C molecule. The resulting A1C molecule is used to measure the average glucose levels in blood over time. ab219049 measures the total Hemoglobin and can recognize both the alpha subunit and A1C modified protein.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hemoglobin subunit alpha also known as alpha-globin is a component of the hemoglobin protein complex which plays an important role in oxygen transport within the blood. Alpha-globin has an approximate molecular weight of 15.1 kDa and is expressed highly in the red blood cells. It is part of the hemoglobin tetramer along with two beta subunits each one containing an iron-bound heme group. Variants of the alpha chain can be studied using hemoglobin peptides or denatured hemoglobin samples. Researchers can further analyze the alpha hemoglobin using methods like SDS-PAGE or alpha ELISA assays.
Biological function summary
Hemoglobin subunit alpha forms an important part of the hemoglobin complex facilitating the binding and release of oxygen molecules as blood circulates in the body. Alpha hemoglobin ensures efficient loading of oxygen in the lungs and unloading in tissues maintaining cellular respiration. The subunit plays a structural role as well stabilizing the hemoglobin tetramer for optimal function. Its ability to carry oxygen depends on the cooperative interaction between its alpha and beta globin counterparts.
Pathways
Hemoglobin subunit alpha operates predominantly within the oxygen transport pathway which is essential to meet the metabolic demands of cells. It also links to pathways involving iron metabolism given its coordination with heme groups. Alpha hemoglobin interacts cooperatively with proteins such as beta-globin to ensure efficient oxygen delivery. This interplay is highlighted when examining hemoglobin biosynthesis and breakdown pathways.
Alpha-globin is linked to conditions like alpha-thalassemia and sickle cell disease. These disorders result from mutations in the hemoglobin alpha or beta subunits leading to imbalanced globin production or abnormal hemoglobin structures. Alpha thalassemia is connected with unequal production of globin chains affecting hemoglobin stability while beta-globin mutations lead to sickle cell disease with altered oxygen delivery. Anti-hemoglobin antibodies might help in researching these conditions allowing a better understanding of molecular changes and potential therapeutic targets.