Abcam, ab219821, Anti-NADH2 antibody [9E12-1B3] - N-terminal

Size: 100µg
Mouse Monoclonal NU2M antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MT-ND2.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:9E12-1B3,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MT-ND2. The exact immunogen used to generate this antibody is proprietary information.P03891

Product details:
Western blot advice
Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

Properties and Storage Information:
Form-Liquid, Purification notes-Purified from hybridoma cell culture supernatant by biochemical fractionation from serum-free medium., Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: 0.87% Sodium chloride, 0.36% HEPES, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NADH2 also known as NADH dehydrogenase 2 is an important enzyme in cellular respiration. It is often referred to as 9e12 in some contexts. It plays a mechanical role in the electron transport chain functioning within the inner mitochondrial membrane where it catalyzes the transfer of electrons from NADH to ubiquinone. This activity contributes to the proton gradient necessary for ATP synthesis. NADH2 has a mass that varies depending on the isoform but typically around 35 kDa. The enzyme is expressed in various tissues notably where high energy demand exists such as in muscle tissues and the liver.
Biological function summary
The enzyme engages in oxidizing NADH thereby producing NAD+ and contributing electrons to the respiratory chain. NADH2 combines with other subunits as part of a larger multi-subunit complex called Complex I in mitochondria. This complex represents a pivotal element in creating the electrochemical gradient used by ATP synthase to produce ATP the main energy currency of the cell. Disturbances in Complex I activity can result in impaired energy production.
Pathways
NADH2 forms an integral component of oxidative phosphorylation and the electron transport chain. It is closely associated with pathways that manage oxidative respiration and energy metabolism within the cell. Important related proteins include cytochrome c and ATP synthase which work downstream of NADH2 in the electron transport chain. Any disruptions in these pathways may significantly influence cellular energy metabolism and overall cellular health.
Malfunctions or deficiencies in NADH2 are linked to mitochondrial diseases notably Leber's hereditary optic neuropathy (LHON) and neurodegenerative diseases such as Parkinson's disease. These connections highlight its critical role in maintaining mitochondrial function and energy production. In the context of these disorders NADH2 often interacts aberrantly with proteins such as complex IV components and other mitochondrially encoded proteins which may exacerbate symptoms through disrupted energy metabolism.