Abcam, ab21990, Anti-Otx1 + Otx2 antibody

Size: 100µg
Anti-Otx1 + Otx2 antibody (ab21990) is a rabbit polyclonal antibody detecting Otx1 + Otx2 in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF, ChIP . Suitable for Chicken, Cow, Human, Mouse, Xenopus laevis . - Over 70 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human, Xenopus laevis, Cow, Chicken,
Applications:IHC-P, IP, ChIP, WB, IHC-Fr, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab21990 is raised against the C-terminal region of the Human Otx2 protein. This product is batch tested in Y79 cell lysate, recombinant Otx1 and overexpressed Otx2. All batches should detect endogenous Otx2, overexpressed Otx2 and recombinant or overexpressed Otx1. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Product details:
What is this antibody validated in?
Anti-Otx1 + Otx2 antibody (ab21990) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Chicken, Cow, Human, Mouse, Xenopus laevis samples.
What is the molecular weight of Otx1 + Otx2?
Anti-Otx1 + Otx2 (ab21990) specifically detects a band for Otx1 + Otx2 (UniProt: P32243) at a molecular weight of 31.6kDa.
Trusted by the scientific community
Anti-Otx1 + Otx2 (ab21990) was first used in a scientific publication in 2006 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-Otx1 + Otx2 (ab21990) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Otx1 and Otx2 also known as orthodenticle homeobox 1 and 2 are homeodomain-containing transcription factors. Otx1 has a molecular mass of approximately 36 kDa and Otx2 about 29 kDa. These proteins are expressed in the developing brain particularly in the forebrain and midbrain regions. In adults their expression continues in regions like the retina and cerebellum. These transcription factors are critical players in neuronal differentiation and brain development.
Biological function summary
Otx1 and Otx2 regulate gene expression during embryonic development. They are part of transcriptional complexes that control the expression of key developmental genes. Their activity influences the formation of neuronal identity and positioning. These proteins help orchestrate the intricate processes needed for proper brain structuring and function serving foundational roles in the early stages of brain formation.
Pathways
Otx1 and Otx2 participate in the Hedgehog and Wnt signaling pathways. These pathways are essential in regulating developmental processes like cell fate determination and patterning in the developing embryo. Within these pathways Otx1 and Otx2 interact with other proteins such as Gli3 in the Hedgehog pathway which further exemplifies their role in developmental signaling networks.
Mutations or dysregulation of Otx1 and Otx2 are associated with disorders such as epilepsy and retinopathy. For example abnormal expression of Otx2 can lead to retinal diseases potentially linking it with proteins involved in retinal health like Crx. Similarly disruptions in Otx1 function have been noted in certain epileptic conditions highlighting the importance of these proteins in maintaining neurological health.