Abcam, ab220408, Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody

Size: 100µL
Rabbit Polyclonal Adenine Nucleotide Translocator 2/ANT 2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC25A5 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SLC25A5 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.P05141,
Specificity:This antibody can also recognise ADP/ATP translocase 2, SLC25A31, SLC25A5 and SLC25A6 due to homology of 93% in immunogen sequence.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Adenine Nucleotide Translocator 2 (ANT 2) and Adenine Nucleotide Translocase 1 (ANT 1) play an important role in mitochondrial energy metabolism. These proteins also referred to as ANT/anti and ANT 1/2 have a mass around 33 kDa each. They are integral components of the inner mitochondrial membrane and widely expressed in various tissues being abundant in those with high-energy demands such as heart and muscle tissues. ANT 2 and ANT 1 facilitate the exchange of ADP and ATP across the mitochondrial membrane critical for cellular energy supply.
Biological function summary
ANT 2 and ANT 1 contribute to maintaining mitochondrial function and integrity. They form part of the larger mitochondrial permeability transition pore complex which plays a role in energy metabolism and apoptosis. By regulating ADP/ATP exchange ANT 2 and ANT 1 ensure efficient energy production aligning cellular energy consumption with metabolic demands. Dysfunctions in their activity can result in impaired ATP synthesis affecting overall cellular viability and function.
Pathways
ANT 2 and ANT 1 participate significantly in critical processes like oxidative phosphorylation and the apoptosis pathway. In oxidative phosphorylation these proteins interact with the F1F0 ATP synthase complex to facilitate ATP production. Within the apoptosis pathway involvement in the opening of the mitochondrial transition pore can trigger programmed cell death. Connections with proteins such as cytochrome c occur in these pathways highlighting their role in energy regulation and cell survival.
Defects in ANT 2 and ANT 1 associate with conditions such as mitochondrial myopathy and dilated cardiomyopathy. Dysfunctional ANT proteins can disrupt mitochondrial homeostasis leading to energy deficiency and cell damage. They connect further to proteins like cytochrome c in apoptosis contributing to disease pathogenesis through altered cell death mechanisms. Investigating these proteins helps understand their role in disease development and offers potential targets for therapeutic intervention.