Abcam, ab221741, Anti-MED25 antibody

Size: 100µL
Rabbit Polyclonal MED25 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MED25 aa 600-700.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MED25 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.Q71SY5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MED25 also known as ARC92 is a subunit of the mediator complex a multiprotein structure that modulates transcription of RNA polymerase II-dependent genes. MED25 has a molecular mass of approximately 80 kDa and shows expression in various tissues including the brain liver and heart. The protein serves as an important link between transcription factors and the basal transcription machinery functioning mechanically as a scaffold facilitating the assembly of transcriptional complexes.
Biological function summary
The MED25 protein plays an essential role in regulating gene expression by interacting with transcription factors. MED25 is part of the larger mediator complex which acts as a bridge to convey regulatory signals from enhancers to promoters. This interaction influences development processes and cellular differentiation by controlling the transcription of specific genes necessary for these functions.
Pathways
MED25 is integral to the transcriptional regulation within the Wnt signaling and TGF-beta pathways. It mediates interactions between transcription factors such as STAT3 and p53 and the transcriptional machinery facilitating signal transduction leading to gene expression changes. These pathways regulate several biological processes including cell growth and apoptotic responses establishing MED25 as an important player in maintaining cellular homeostasis.
MED25 has been implicated in neurological disorders such as Charcot-Marie-Tooth disease. MED25 mutations have been associated with this peripheral neuropathy affecting nerve conduction. Additionally disruptions in MED25 interactions with the transcription machinery have connections to cancer development since its role in pathways like TGF-beta involves proteins like SMAD3 that regulate cell proliferation and apoptosis. Understanding MED25's function and interactions offers potential therapeutic avenues for these diseases.