Abcam, ab222953, Recombinant mouse Alanine Transaminase protein (Active)

Size: 20µg
Recombinant mouse Alanine Transaminase protein (Active) is a Mouse Full Length protein, in the 1 to 496 aa range, expressed in Escherichia coli, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:FuncS, Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity is > 100 units/mg, and is defined as the amount of enzyme that converts 1 μmole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37°C.,
Accession:Q8QZR5,
Animal free:No,
Carrier free:No,
Species:Mouse,
Storage buffer:pH: 7.4Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.
Biological function summary
Alanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.
Pathways
The role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.
Alanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.