Abcam, ab223044, Anti-RAB27A antibody

Size: 50µg / 50µL
Rabbit Polyclonal RAB27A antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAB27A.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human RAB27A.P51159

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB27A also known as ras-related protein Rab-27A is a small GTPase that plays a role in intracellular vesicle trafficking. It has a molecular mass of approximately 25 kDa. RAB27A localizes in many cell types with higher expressions in secretory cells such as melanocytes and cytotoxic T lymphocytes. It primarily functions in the regulation of vesicle docking and fusion processes with target membranes facilitating the transport of contents such as enzymes and other proteins to specific sites within the cell.
Biological function summary
This protein regulates the exocytosis processes in certain secretory cells. RAB27A partners with effectors through its GDP-bound and GTP-bound states forming protein complexes to mediate the movement and anchoring of secretory granules. Melanocytes depend on RAB27A to control the distribution of melanosomes which are organelles containing pigment melanin. In immune cells RAB27A regulates the secretion of lytic granules significantly impacting the immune response.
Pathways
This protein is involved in the melanogenesis pathway and the lytic granule exocytosis pathway. In the melanogenesis pathway RAB27A works closely with proteins such as Melanophilin and Myosin Va to control melanosome transport. In the exocytosis pathway in immune cells RAB27A interacts with proteins like UNC13D which are vital for the release of cytotoxic molecules. By controlling these pathways RAB27A influences pigment formation in skin and hair as well as immune defense mechanisms.
Mutations in the RAB27A gene are associated with Griscelli syndrome type 2 a disorder characterized by partial albinism and immune system defects. This syndrome arises from impaired melanosome transport due to dysfunctional RAB27A and its associated proteins such as Melanophilin and Myosin Va. Additionally defects in RAB27A also relate to hemophagocytic lymphohistiocytosis type 2 where impaired function in cytotoxic T lymphocytes reduces immune response effectiveness leading to excessive inflammation.