Abcam, ab224222, Anti-SMEK2 antibody

Size: 100µL
Rabbit Polyclonal SMEK2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPP4R3B aa 650-750.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PPP4R3B aa 650-750. The exact immunogen used to generate this antibody is proprietary information.Q5MIZ7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMEK2 also known as protein phosphatase 4 regulatory subunit 3B (PPP4R3B) is a regulatory subunit of the protein phosphatase 4 (PP4) complex. SMEK2 exhibits a molecular mass of about 106 kDa. It is distributed in various tissues with notable expression in the brain and skeletal muscle. As part of the PP4 complex its primary role is to guide and regulate the specific functions of the catalytic subunits of the complex.
Biological function summary
SMEK2 regulates cellular processes by modulating phosphatase activity. It acts as part of the PP4 complex which includes the PP4 catalytic subunit and other regulatory subunits. This complex plays a role in diverse biological functions such as cellular response to DNA damage and cell division. By affecting dephosphorylation events SMEK2 influences cellular signaling allowing for precise cellular responses under varying conditions.
Pathways
SMEK2 significantly impacts the DNA damage response and cell cycle regulation pathways. It interacts closely with proteins such as PP4C the catalytic subunit of the PP4 complex and has connections to key checkpoint regulators in these pathways. Such interactions ensure accurate DNA repair and proper progression through cell cycle phases. Changes in SMEK2’s functionality or expression can disrupt these pathways leading to improper cellular responses.
Altered SMEK2 activity or expression has been associated with cancer and neurodegenerative diseases. In cancer SMEK2 along with PP4C influences tumor suppressor pathways by regulating processes like DNA damage repair. Disruption in these interactions can lead to uncontrolled cell proliferation. Additionally abnormalities in SMEK2 have been linked to Alzheimer's disease possibly due to its involvement in neuronal signaling pathways and response to cellular stress.