Abcam, ab224321, Anti-HCCS antibody

Size: 100µL
Rabbit Polyclonal HCCS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HCCS aa 50-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human HCCS aa 50-250. The exact immunogen used to generate this antibody is proprietary information.P53701

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The holocytochrome c-type synthase (HCCS) also known as cytochrome c heme lyase attaches to the inner mitochondrial membrane. It catalyzes the covalent attachment of heme to apocytochrome c providing an essential step in cytochrome c maturation. HCCS with a mass around 30 kDa expresses predominantly in tissues with high energy demand such as heart and muscle tissue. The protein’s location suggests its significant role in aerobic energy production processes.
Biological function summary
Holocytochrome c-type synthase facilitates proper electron transport chain function by enabling cytochrome c activity. It operates as part of a larger mitochondrial complex critical for oxidative phosphorylation. Without HCCS the conversion of apocytochrome c to its holo form fails impacting the electron transport chain's efficiency and leading to impaired energy production.
Pathways
Holocytochrome c-type synthase plays an important role in the mitochondrial electron transport chain and apoptotic pathways. Through its function HCCS influences both the generation of ATP and regulation of programmed cell death. It interacts closely with cytochrome c an essential component in these pathways emphasizing its integrative role in cellular respiration and apoptosis regulation.
Disturbances in HCCS activity link to disorders such as microphthalmia with linear skin defects syndrome (MLS) and certain mitochondrial abnormalities. These involve alterations in the mitochondrial functionalities where HCCS interacts with cytochrome c and related proteins. Such relationships highlight the critical impact of HCCS on cellular health and stress the importance of its proper functioning in preventing disease.