Abcam, ab225596, Recombinant Mycobacterium tuberculosis Ag85 protein (His tag)

Size: 100µg
Recombinant Mycobacterium tuberculosis Ag85 protein (His tag) is a Mycobacterium tuberculosis H37Rv Full Length protein, in the 46 to 340 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P9WQN9,
Animal free:No,
Carrier free:No,
Species:Mycobacterium tuberculosis H37Rv,
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein Mycobacterium tuberculosis Ag85 also called Ag85 complex or Ag-85 consists of a series of related proteins secreted by Mycobacterium tuberculosis including Ag85A Ag85B and Ag85C. This protein family plays a central role in the pathogen's ability to synthesize mycolic acids. The Ag85 complex has a molecular mass of around 30 to 32 kDa and is abundantly expressed on the surface of the Mycobacterium tuberculosis cell wall.
Biological function summary
The Ag85 complex facilitates the synthesis and transfer of mycolic acids essential constituents of the mycobacterial cell wall's lipid-rich structure. The Ag85 proteins participate in forming this barrier allowing Mycobacterium tuberculosis to resist host immune defenses. This functionality is linked to its involvement in the mycolyltransferase enzyme system which operates in collaboration with several other proteins necessary for full cell wall biosynthesis.
Pathways
The Ag85 complex significantly contributes to the biosynthetic route of mycolic acids an integral component of mycobacterial cell walls. This pathway includes interactions with other proteins like Pks13 and Mycobacterial arabinosyltransferase (EmbB) both important for the complex architectural integrity and viability of the mycobacterial cell wall. This biosynthesis pathway ensures proper assembly and maintenance of the cell envelope enhancing the persistence of the bacteria.
The Ag85 proteins are critical in the pathogenesis of tuberculosis where their activity supports the survival of the bacteria within host macrophages. Ag85 complexes are considered potential targets for diagnostic and therapeutic applications against tuberculosis. Additionally in leprosy another disease caused by mycobacteria proteins of the Ag85 complex have shown cross-reactivity emphasizing their significant role in bacterial viability and immune response evasion.