Abcam, ab225742, Anti-Integrin beta antibody [EPR20825] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Integrin beta 3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20825,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins: beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.

Product details:
ab225742 is the carrier-free version of
ab210515
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Integrin beta also known as ITGB is a mechanical transducer involved in cell adhesion and signaling. Integrins are heterodimeric proteins consisting of alpha and beta subunits. The human integrin beta subunit has a molecular mass of about 90-110 kDa and it exists in several isoforms such as ITGB1 ITGB2 ITGB3 among others. These integrin beta subunits are widely expressed across various tissues including epithelial cells endothelial cells and immune cells. They mediate interactions between the extracellular matrix and the cytoskeleton inside the cell influencing cellular responses like movement and survival.
Biological function summary
Integrin beta participates actively in mediating cell-extracellular matrix (ECM) interactions as part of the integrin complex. By forming dimers with different alpha subunits integrin beta plays an important role in cellular processes such as differentiation proliferation and apoptosis. The ability of integrin beta subunits to interact with different ECM proteins such as fibronectin and collagen allows them to orchestrate tissue-specific functions. Furthermore integrin beta influences signal transduction pathways leading to activation of intracellular signaling molecules like focal adhesion kinase (FAK) and Src family kinases.
Pathways
Integrin beta significantly impacts cell signaling and communication. It is central to the focal adhesion pathway where it influences the assembly of focal adhesion points that connect cells to the ECM. This connection supports signal cascades that involve Ras-MAPK and PI3K-Akt pathways important for regulating cell proliferation and migration. Integrin beta also interacts with proteins like talin and kindlin enhancing its signaling capabilities and stability at the cell surface thereby affecting cytoskeletal dynamics and cellular motility.
Integrin beta is associated with a range of pathological conditions. Abnormal integrin beta function contributes to cancer progression and metastasis as its altered expression can affect cell adhesion and invasion. Additionally integrin beta dysfunction is implicated in inflammatory diseases like multiple sclerosis where it interacts with proteins such as VCAM-1 to facilitate immune cell infiltration into the central nervous system. Understanding these interactions and their effects on disease development remains a focus for therapeutic interventions targeting integrin beta and its pathways.