Abcam, ab225982, Recombinant Gingipain R1 protein (His tag)

Size: 100µg
Recombinant Gingipain R1 protein (His tag) is a Porphyromonas gingivalis Fragment protein, in the 228 to 720 aa range, expressed in Yeast, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Yeast,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P28784,
Animal free:No,
Carrier free:No,
Species:Porphyromonas gingivalis,
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Gingipain R1 also known as RgpA is a cysteine protease enzyme with a molecular mass of approximately 95 kDa. It is expressed predominantly in the outer membrane of Porphyromonas gingivalis a bacterium important in periodontal disease. Gingipains including Gingipain R1 play an essential role in bacterial virulence and are known for their cleavage specificity towards Arg-X peptide bonds. They contribute significantly to the degradation of host proteins and are key factors in the processing of bacterial proteins facilitating the survival and pathogenicity of the bacterium.
Biological function summary
Gingipain R1 acts as a potent virulence factor of Porphyromonas gingivalis by aiding the invasion and destruction of periodontal tissues. It is known to be part of a larger proteolytic complex which enhances its enzymatic activity and stability. Gingipains degrade extracellular matrix proteins and modulate the host immune response allowing the bacteria to evade immune mechanisms. This protease can activate inflammatory cytokines through the cleavage of cell surface components amplifying inflammatory processes in the host tissue.
Pathways
Gingipain R1 contributes significantly to the coagulation pathway by modulating fibrinogen and fibrin. It also affects the complement pathway indirectly inhibiting complement-mediated immune responses via degradation of complement proteins. Gingipain R1 interaction with other bacterial proteins such as hemagglutinin further strengthens its functional role in these pathways facilitating pathogenic interactions with the host.
Gingipain R1 is closely associated with periodontitis due to its destructive capabilities on periodontal tissue and its role in exacerbating inflammation. It is also implicated in systemic disorders like cardiovascular disease likely due to its influence on inflammation and immune response modulation. Its activity closely links with other proteases such as Gingipain K (Kgp) which collectively enhance the pathogenic potential of Porphyromonas gingivalis by contributing to the bacterial evasion of host defenses and promotion of tissue damage.