Abcam, ab226249, Anti-Calpastatin antibody

Size: 100µL
Rabbit Polyclonal Calpastatin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Calpastatin aa 600-650.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Calpastatin aa 600-650. The exact immunogen used to generate this antibody is proprietary information.P20810

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab226249 was affinity purified using an epitope specific to Calpastatin immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Calpastatin is an endogenous inhibitor of calpain proteases with popular alternate names including CAST. The mass of calpastatin is approximately 70 kDa. It is expressed in various tissues throughout the body such as the brain heart and muscle. Calpastatin regulates the activity of the calpain family of cysteine proteases by binding to their catalytic domains. By modulating calpain activity calpastatin plays a role in several cellular processes including cytoskeletal remodeling and signal transduction.
Biological function summary
Calpastatin regulates intracellular protein degradation by inhibiting calpain activity. This inhibition prevents uncontrolled proteolysis of specific substrate proteins. Calpastatin is not part of a larger protein complex but interacts independently with multiple isoforms of calpain. It helps maintain cellular homeostasis by influencing autophagy apoptosis and cell migration integrating signals that drive various physiological processes.
Pathways
Calpain activity modulation through calpastatin takes place in several key biological pathways including apoptosis and the Wnt signaling pathway. Calpain-mediated cleavage affects cell survival and death by modifying Bcl-2 family proteins. In the Wnt signaling pathway calpastatin regulates calpain which influences β-catenin degradation therefore affecting cell proliferation and differentiation pathways. These interactions highlight calpastatin’s central role in managing fundamental cellular events.
Uncontrolled calpain activity due to insufficient calpastatin levels relates to neurodegenerative diseases such as Alzheimer's disease and muscular dystrophies. In Alzheimer's disease reduced calpastatin levels may lead to excessive calpain activity resulting in tau protein hyperphosphorylation and neurofibrillary tangles. Calpastatin-related calpain dysregulation also contributes to muscle cell degeneration seen in muscular dystrophies by affecting structural proteins like dystrophin. These connections highlight the critical balance that calpastatin maintains in cellular processes pertinent to human health.