Abcam, ab227512, Anti-GATA4 antibody

Size: 100µL
Rabbit Polyclonal GATA4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GATA4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human GATA4. The exact immunogen used to generate this antibody is proprietary information.P43694

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GATA4 also known as GATA-binding protein 4 is a transcription factor that plays a significant role in gene expression regulation. It weighs approximately 44 kDa. This protein expresses mainly in cells of the heart gonads and liver. GATA4 functions through binding to the GATA sequence on DNA regulating various genes. The protein is well known for its involvement in cardiac development and function.
Biological function summary
GATA4 interacts with other transcription factors and co-factors to regulate developmental processes. It often forms complexes with proteins such as NKX2-5 and TBX5 influencing the expression of cardiac-specific genes. These interactions lead to tissue-specific gene expression control important in the differentiation and functioning of heart cells. GATA4 is also involved in the regulation of genes responsible for cell growth and apoptosis contributing to tissue homeostasis.
Pathways
GATA4 is a notable component of cardiac development and cellular differentiation pathways. It plays an integral role in the Wnt signaling pathway influencing heart development through gene transcription modification. GATA4 also collaborates with proteins such as MEF2 and BMP2 coordinating cardiac muscle differentiation. These pathways highlight its pivotal role in proper cardiovascular formation and function.
GATA4 mutations and dysregulation have links to congenital heart defects such as atrial septal defect (ASD). This condition associates with altered gene expression during heart development leading to structural abnormalities. GATA4 also relates to conditions like hypertrophic cardiomyopathy where its interaction with proteins like MYH7 suggests a complex network influencing cardiac hypertrophy. Understanding these connections is essential for developing targeted therapeutic strategies.