Abcam, ab228874, Anti-EIF5 antibody

Size: 100µL
Rabbit Polyclonal EIF5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Eukaryotic translation initiation factor 5.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Eukaryotic translation initiation factor 5. The exact immunogen used to generate this antibody is proprietary information.P55010

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The eukaryotic translation initiation factor 5 (EIF5) plays an important role in the initiation phase of protein synthesis. EIF5 also known as eIF-5 is a protein that exhibits a molecular weight of approximately 49 kDa. It functions as a GTPase-activating protein that triggers the hydrolysis of GTP bound to the eukaryotic initiation factor 2 (eIF2) an essential step for the formation of the 80S ribosome complex during translation. EIF5 expression occurs widely across various tissues indicating its necessity for basic cellular functions.
Biological function summary
EIF5 facilitates the translation initiation process which is critical for protein synthesis. It is an integral component of the multifactor eIF2 complex that ensures the accuracy of start codon recognition in mRNA. This complex assembly involves the association with other initiation factors like eIF1 and eIF3 orchestrating mRNA scanning and the selection of the start site for protein synthesis. EIF5's role in regulating protein biosynthesis links it to the maintenance of cellular homeostasis.
Pathways
EIF5 serves a critical part in the translation initiation pathway. This pathway is essential for general protein biosynthesis directly impacting cellular growth and proliferation. It closely collaborates with related factors like eIF2 and eIF2B within this pathway to modulate translation in response to cellular stress and nutrient availability. Additionally EIF5 intersects the mTOR signaling pathway where it interacts with proteins that coordinate cell growth and metabolism under various physiological conditions.
EIF5 alterations can lead to disruptions in protein synthesis connecting it to certain human diseases like cancer. Some studies indicate a link between EIF5 dysregulation and tumorigenesis as faults in translation regulation can drive abnormal cell proliferation. Beyond cancer EIF5 may also intersect with neurodegenerative diseases given the translation regulation's importance in neuronal function. It can associate with other factors such as eIF2α wherein heightened EIF5 activity influences stress responses pivotal in disease contexts.