Abcam, ab229419, Human Fibrinogen ELISA Kit, Fluorescent

Size: 1 x 96Tests
Human Fibrinogen ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Fibrinogen with a sensitivity of 29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
Detection method:Fluorescent,
Sample types:Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 29 pg/mL,
Range:15.63 - 64000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Fibrinogen ELISA Kit, Fluorescent ab229419 is a rapid single-wash 90-min sandwich ELISA to measure Human Fibrinogen in serum, heparin plasma, citrate plasma, EDTA plasma, cell culture supernatant, saliva, urine. This CatchPoint SimpleStep sensitivity is 29 pg/mL.
How the assay works
Human Fibrinogen CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission. This kit is optimised for Molecular Devices Readers: If using a Molecular Devices' plate reader supported by SoftMax
Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Fibrinogen ELISA Kit, Fluorescent ab229419 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a fluorescent plate reader at 530/570/590 nm Excitation/Cutoff/Emission.
Colorimetric version
This kit is also available with a colorimetric readout as Human Fibrinogen ELISA Kit - high sensitivity
ab241383
Both the fluroescent and colorimetric version share the same antibody pair and protein standard.
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Anti-Fibrinogen antibody [EPR19765-189] - BSA and Azide free (Capture)
ab244636
- Anti-Fibrinogen antibody [EPR19765-63] - BSA and Azide free (Detector)
ab244897

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C