Abcam, ab230509, Anti-MT-ND5 antibody

Size: 200µL
Rabbit Polyclonal MT-ND5 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MT-ND5 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human MT-ND5 conjugated to Keyhole Limpet Haemocyanin.P03915

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MT-ND5 also known as NADH-ubiquinone oxidoreductase chain 5 is a subunit of mitochondrial complex I found in the inner mitochondrial membrane. This protein weighing approximately 74 kDa plays a central role in the electron transport chain facilitating the transfer of electrons from NADH to ubiquinone. Expression of MT-ND5 occurs ubiquitously in tissues with high energy demands such as the heart brain and skeletal muscle where efficient ATP production is essential.
Biological function summary
MT-ND5 functions as an integral component of mitochondrial complex I which is the first enzyme complex in the electron transport chain. By participating in this complex MT-ND5 contributes to the generation of a proton gradient across the inner mitochondrial membrane driving ATP synthesis through oxidative phosphorylation. Malfunction of MT-ND5 impairs energy production which can lead to a cascade of cellular energy deficiencies given its role within the complex.
Pathways
MT-ND5 associates with the oxidative phosphorylation pathway. It is important for maintaining aerobic energy production by linking NADH oxidation with ubiquinone reduction. Other proteins like MT-ND1 and MT-ND4L also participate in electron transport chain pathways working alongside MT-ND5 to ensure effective electron transfer and mitochondrial respiration. Its function within these pathways highlights its importance in metabolic regulation.
MT-ND5 mutations have associations with mitochondrial disorders such as Leigh syndrome and mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS). Defective MT-ND5 affects the electron transport chain leading to energy metabolism disruption in affected tissues. These disorders often involve other mitochondrial proteins such as MT-ND1 where combined mutations further exacerbate the dysfunction of mitochondrial capacity and highlight the profound impact on human health.