Abcam, ab231080, Anti-C3b / iC3b antibody [1H8]

Size: 100µg
Mouse Monoclonal C3 antibody. Suitable for ELISA, WB and reacts with Human samples. Cited in 5 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1H8,
Isotype:IgG2,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
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Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C3b protein also known as complement component 3b plays a significant role in the immune system aiding in the clearance of pathogens. It is a large glycoprotein with a mass of approximately 180 kDa. C3b is expressed mainly in the liver and released into the bloodstream as part of the complement system. Upon activation from its precursor C3 it becomes involved in opsonization enhancing the phagocytosis of pathogens by marking them for destruction by immune cells.
Biological function summary
C3b acts as a critical mediator in the immune system by forming complexes with other complement proteins. It is part of the alternative and classical pathways of the complement system. C3b can also convert itself into iC3b after cleavage by factor I and iC3b continues to facilitate phagocytosis but with reduced ability to promote further complement activation. This conversion ensures homeostasis and regulation preventing overactivation of the immune system.
Pathways
C3b participates in both the classical and alternative pathways of the complement system. It associates with proteins such as factor B and factor D to form the C3bBb complex in the alternative pathway acting as a C3 convertase that amplifies response. In the classical pathway C3b enhances the activity of C5 convertase facilitating the formation of the membrane attack complex. These pathways are vital in maintaining immune surveillance and response to infections.
Dysfunction or dysregulation of C3b activity links to autoimmune diseases and infectious diseases. For example atypical hemolytic uremic syndrome (aHUS) involves uncontrolled complement activation due to mutations affecting proteins like C3b. In such conditions C3b and related proteins such as factor H become therapeutic targets to modulate the complement system's overactivation. Studying the role of C3b in these diseases provides insight into potential interventions and novel treatments to manage complement-related pathologies.