Abcam, ab234431, Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17]

Size: 20µL / 100µL / 1mL
Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431) is a rabbit monoclonal antibody detecting Slow Skeletal Myosin Heavy chain in IHC-P, IHC-Fr, mIHC . Suitable for Human, Mouse, Rat . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22697-17,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:mIHC, IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR22697-17] also available for your convenience: ab234431, Carrier free -
ab255278
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The slow skeletal myosin heavy chain also known as anti-slow or anti-sluggish is an essential protein component of muscle tissue. This protein is identified as MYH7 chain or type D chain with a molecular mass around 220 kDa. It is predominantly expressed in slow-twitch muscle fibers which are found in skeletal muscles responsible for enduring low-intensity (endurance) activities. Myosin staining techniques often highlight the distribution and density of these fibers in muscle tissues providing insights into fiber-type composition.
Biological function summary
This myosin heavy chain participates in muscle contraction by interacting with actin filaments forming cross-bridges through ATP-dependent mechanisms. As a part of the sarcomeric structure MYH7 works within the thick filament complex contributing to the contractile force of slow-twitch muscles. This particular myosin isoform is important for maintaining muscle stamina and efficiency over prolonged activities providing the necessary mechanical support for the generation of force at a lower contraction speed compared to fast-twitch fibers.
Pathways
The slow skeletal myosin heavy chain is integral to the calcium signaling pathway that regulates muscle contraction. This protein interacts with troponin and tropomyosin to modulate the actin-myosin interaction in a calcium-dependent manner. Additionally it plays a role in the heart muscle contraction pathway linking it to proteins such as actin which is important for cardiac function and rhythmic contraction.
Slow skeletal myosin heavy chain mutations are linked to cardiomyopathy particularly hypertrophic cardiomyopathy and dilated cardiomyopathy. These conditions compromise heart muscle function leading to symptoms such as fatigue and shortness of breath. The MYH7-associated disorder is often observed alongside alterations in other sarcomeric proteins like troponin T which further disrupts muscle contraction and cardiac output. Understanding these connections can aid in developing interventions and treatments for muscle-related diseases.