Thermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics

From accurate protein quantitation to western blotting, affinity purification, crosslinking, and MS-based proteomics, this curated collection brings you high-performing Thermo Fisher reagents trusted in everyday protein biology. Explore by use case, compare specs, and jump straight to validated kits and chemistries.

What’s in This Collection (by product family)

This collection is organized to help you quickly find the right reagent for your workflow while keeping SKUs transparent for purchasing and compliance. Below are the major families you’ll find and representative examples.

• Protein quantitation assays — BCA (e.g., Pierce™ BCA Protein Assay Kit, Cat. 23225), Micro BCA (23227), 660 nm assay reagent (22660) and complete kit (22662).

• Cell lysis & inhibitors — RIPA Lysis Buffer (89900); Halt™ Protease Inhibitor Cocktail 100×, EDTA-free (87785) or EDTA-containing (87786); combined Protease/Phosphatase inhibitor (78440).

• Electrophoresis & western blotting reagents — SuperSignal™ West Pico PLUS ECL substrate (34580); West Femto substrate (34095); PVDF transfer membranes 0.45 µm roll (88518); Nitrocellulose membranes 0.45 µm (88018); PageRuler™ Plus Prestained Protein Ladder (26619).

• Affinity tools & cleanup — Protein A/G Magnetic Beads (88802); HisPur™ Ni-NTA Resin (88221); Zeba™ Spin Desalting Columns 7K MWCO (89882, 89890).

• Conjugation & crosslinking — EZ-Link™ Sulfo-NHS-Biotin (21217); EZ-Link™ NHS-PEG4-Biotin (21330); BS3 crosslinker (21580, 21586).

• Proteomics sample prep — Pierce™ Trypsin Protease, MS-grade (90057); TMTpro™ 16-plex labels (A44520) and 18-plex (A52045).

Compatibility & Specs (quick reference)

Choosing reagents that match your sample matrix and detection method saves time downstream. Use this compact view to confirm compatibility before you order, then drill down into datasheets as needed.

Applications & Workflows (four quick-start cards)

These simple, dependable flows help you reduce variables and keep data consistent across experiments. Follow the steps and swap in the exact SKUs listed above as needed.

A. Western blot — from lysate to signal

1. Lyse in RIPA (89900) + Halt inhibitors (87785).

2. Quantify (BCA 23225 or 660 nm 22660).

3. Run SDS-PAGE; transfer to PVDF (88518) or Nitrocellulose (88018).

4. Detect with West Pico PLUS (34580) or West Femto (34095).

B. IP/Co-IP — enrich your target

1. Pre-clear lysate; bind to Protein A/G Magnetic Beads (88802).

2. Wash; elute in mild buffer.

3. Analyze by SDS-PAGE and western blot as above.

C. His-tag purification + cleanup

1. Bind to HisPur Ni-NTA Resin (88221) under native or denaturing conditions.

2. Elute with imidazole; exchange buffer on Zeba columns (0.5–2 mL: 89882, 89890).

3. Quantify and proceed to downstream assays. 

D. Proteomics — digest and multiplex

1. Reduce/alkylate; digest with Pierce Trypsin MS-grade (90057).

2. Label peptides with TMTpro 16-plex (A44520) or 18-plex (A52045).

3. Combine, fractionate, and analyze by LC-MS/MS.

Practical Tips (to improve first-pass success)

Before diving in, a few small adjustments can materially improve reproducibility and signal-to-noise across workflows. Keep these suggestions handy as you plan and document your runs.

• Match assay chemistry to matrix. Use 660 nm when ionic detergents or strong reducers are unavoidable; add IDCR if needed to extend tolerance further.

• Pick membrane by size. 0.45 µm PVDF or nitrocellulose suits most proteins; consider 0.2 µm for <20 kDa targets to reduce blow-through.

• Choose substrate to fit abundance. West Pico PLUS for routine expression; West Femto for very low targets to avoid overexposure.

• Protect proteins from the start. Add Halt inhibitors at lysis and keep samples cold to prevent proteolysis.

• For His-tag purifications, wash with moderate imidazole (20–40 mM) to lower nonspecific binding before elution.

• Use desalting steps after labeling, crosslinking, or buffer changes to boost downstream assay performance.

• Document lot and SKU in your ELN alongside dilution factors; this speeds audits and method transfer.

FAQs

Simple answers to the questions we hear most often, with links to primary documentation for deeper reading.

Q1: When should I choose the 660 nm assay instead of BCA?
A: If your samples contain ionic detergents or high reducer levels, the 660 nm assay is generally more tolerant (and can be paired with IDCR). Use BCA when the matrix is simpler and you want the broad dynamic range with matched standards.

Q2: PVDF or nitrocellulose—what’s best for my blot?
A: PVDF offers higher protein binding and durability for reprobing; nitrocellulose is fast and gives low background. Both 0.45 µm formats work for most proteins.

Q3: Which ECL substrate should I start with?
A: Begin with West Pico PLUS for typical targets; move to West Femto if expression is very low or if you need the highest sensitivity without extending exposure times.

Q4: Can I use HisPur Ni-NTA under denaturing conditions?
A: Yes. HisPur Ni-NTA is compatible with native or denaturing workflows; just ensure imidazole and pH are optimized.

Q5: What’s a standard proteomics prep from cells to LC-MS?
A: Lyse with inhibitors, reduce/alkylate, digest with Pierce Trypsin (MS-grade), clean up, and multiplex with TMTpro labels before LC-MS/MS.

CTA — Build your kit and get reliable results

Ready to assemble your workflow? Add the SKUs above to your cart, or reach out for a tailored bill of materials by application (western blot, IP, IMAC, or proteomics). If you share your sample type and detection system, we’ll recommend a bundle that fits your budget and timeline.