Waters, 176003712, Glycoworks RapiFluor-MS N-Glycan Starter Kit-24 sample

Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive. This starter kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-up Module, GlycoWorks Sample Collection Module, as well as the ACQUITY UPLC Glycan BEH Amide, 1.7 µm, 2.1 x 150 Column and Mobile-Phase Concentrate: Ammonium Formate. It contains materials to process 24 samples in a 3x8 format. The kit contains: GlycoWorks Deglycosylation Module: (1) 0.035 ml/vial Rapid PNGaseF Enzyme (1) 0.25 ml/vial Glycoworks Rapid Buffer (1) 3 vials of 3 mg/vial RapiGest™ SF Surfactant (1) Intact mAb Mass Check Standard *Shipped in a separate box GlycoWorks RapiFluor-MS Labeling Module: (3) 9 mg/vial RapiFluor-MS Reagent (3) 1 mL/vial Reagent Solvent (Anhydrous DMF) GlycoWorks RFMS Clean-up Module: (4) 5 mL/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7) (4) 8 mL/vial Sample Diluent (DMF/ACN Mixture) (1) GlycoWorks HILIC µElution Plate GlycoWorks Sample Collection Module: (15) 1 mL tubes for deglycosylation and labeling reaction (15) 600 µL tube inserts for SPE eluate collection (15) Cap strips with slits for direct injection (1) 1 mL round collection tray (1) Waste tray Extras: (1) 5050 mM Ammonium Formate Solution—Glycan Analysis (186007081) (1) ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7μm, 2.1 x 150 mm (186004742) (1) RapiFluor-MS Glycan Performance Test Standard This kit includes p/n: 186010922 which is a reduced packaging combination of the RFMS Labelling, Clean-up, and Sample Collection modules. p/n: 186010922 cannot be purchased separately and will only be sold as part of a kit.

Specifications
Number of Analysis: 24
Type: Sample Preparation Workflow
Packages per kit: 7
Separation Mode: HILIC
Detection Method: MS, MS/MS, UV, HRMS
Storage Condition: Refer to the storage recommendations of each sub-package
System Type: LC-MS
Shelf Life: Refer to the storage recommendations of each sub-package
UNSPSC: 41116104
Application: Glycan
Brand: GlycoWorks
Product Type: Application Kits
Units per Package: 1 pk

Additional Information
GlycoWorks RapiFluor-MS N-Glycan Starter Kit-24 sample As part of the effort to innovate biotherapeutic analysis, Waters has re-envisioned the workflow for released N-Glycan analysis using the novel reagent RapiFluor-MS. This new model makes it possible to get the ionization capability necessary to maximize the information generated during MS detection. With speedier deglycosylation and streamlined labeling, the new process stops you from having to choose between fast labeling and sensitivity in MS. By creating a 3-step process that can be done in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Start Kit offers unique chemical attributes for increased fluorescence quantification and supreme mass spectral responses. The starter kit includes enough material for 24 samples in a 3x8 format. If needed, a GlycoWorks RapiFluor-MS N-Glycan Refill Kit-24 sample can be purchased. The kit includes the following lab equipment : GlycoWorks Deglycosylation Module: (1) 0.035 ml/vial Rapid PNGaseF Enzyme; (1) 0.25 ml/vial Glycoworks Rapid Buffer; (1) 3 vials of 3 mg/vial RapiGest™ SF Surfactant; (1) Intact mAb Mass Check Standard *Shipped in a separate box GlycoWorks RapiFluor-MS Labeling Module: (3) 9 mg/vial RapiFluor-MS Reagent; (3) 1 mL/vial Reagent Solvent (Anhydrous DMF) GlycoWorks RFMS Clean-up Module: (4) 0.5 ml/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7); (4) 3 ml/vial Sample Diluent (DMF/ACN Mixture); (1) GlycoWorks HILIC µElution Plate GlycoWorks Sample Collection Module: (15) 1 mL tubes for deglycosylation and labeling reaction; (15) 600 µL tube inserts for SPE eluate collection; (15) Cap strips with slits for direct injection; (1) 1 mL round collection tray; (1) Waste tray Extras: (1) 5050 mM Ammonium Formate Solution—Glycan Analysis (186007081); (1) ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7μm, 2.1 x 150 mm (186004742) ; (1) RapiFluor-MS Glycan Performance Test Standard

FAQ
What Is Delycosylation? Glycosylation is a reaction in which a carbohydrate (ie, a glycosyl donor) is attached to a hydroxyl or other functional group of another molecule (ie, a glycosyl acceptor). Essentially, this is when a sugar entity is attached to glycogen. N-linked glycans attached to a nitrogen, requiring participation of a special lipid.
Thus, deglycosylation is the removal of a sugar entity from glycogen, especially from a glycoprotein. A variety of enzymes can be used to remove the glycans from the proteins or to remove some part of the sugar chain.