Abcam, ab133741, Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker

Size: 20µL / 100µL / 1mL
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) is a rabbit monoclonal antibody detecting Lamin B1 in Western Blot, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 180 publications -  Recommended for methanol-fixed samples in ICC only. Use ab229025 for compatibility with both methanol and PFA.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR8985(B),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has been cited over 189 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality manufacturing and validation processes ensure Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has been confirmed by testing in knockout samples.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) has 14 independent reviews from customers.
Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab133741) specifically detects Lamin B1 (UniProt ID: P20700; Molecular weight: 66kDa) and is sold in a convenient trial size to enable initial testing (20 uL) and larger sizes for subsequent scaling up experiments (100 uL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR8985(B) -
ab22797
Antibody clone EPR8985(B) is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, HRP, Alexa Fluor
ab19416
, ab19418, ab19419,
ab216723
Lamin B1/LMNB1/LMNB is a critical component of the nuclear lamina, playing a vital role in chromatin organization and nuclear stability. Utilizing our lmnb1 antibody can significantly enhance the detection and study of Lamin B1. Research indicates that mutations in Lamin B1 are linked to demyelinating diseases, such as adult-onset autosomal dominant leukodystrophy (ADLD), highlighting its importance in maintaining neural health
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lamin B1 also known as LMNB1 is a protein that plays an important role in the structure of the nuclear lamina a dense fibrillar network inside the nucleus of cells. This protein belongs to the lamin family and has an approximate molecular weight of 66 kDa. Lamin B1 is expressed in nearly all types of cells acting as a structural component by interacting with chromatin and anchoring the nuclear envelope. It contributes to regulating nuclear stability and mechanical support which is essential for proper cell function.
Biological function summary
Lamin B1 serves many important functions by providing structural integrity and regulating gene expression through chromatin organization. Part of the nuclear lamina complex Lamin B1 interacts with other lamin proteins such as Lamin A and Lamin C forming a mesh-like structure that supports the nuclear envelope. This protein also affects cellular processes such as DNA replication RNA transcription and cell division indicating its broad impact on various cellular activities.
Pathways
Lamin B1 plays an important role in gene expression regulation and cell cycle control. It interacts with pathways involved in chromatin remodeling influencing the access of transcription factors to DNA which affects gene expression profiles. Lamin B1 associates with related proteins such as Lamin A/C and other nuclear matrix proteins to maintain cellular architecture stability and normal cell function. This organization is vital to ensure the accuracy of cellular processes and prevent anomalies during cell division.
Mutations or altered expression of Lamin B1 have been associated with several pathological conditions. One noteworthy disorder is autosomal dominant adult-onset leukodystrophy where Lamin B1 expression levels are abnormal. This protein also connects to multiple sclerosis through its role in maintaining nuclear architecture. Additionally changes in Lamin B1 expression levels can influence pathways involving Lamin A/C and potentially lead to other laminopathies highlighting its influence in maintaining cellular health and contributing to disease development.