Abcam, ab217344, Anti-CD8 alpha antibody [EPR21769]

Size: 100µL / 1mL
Anti-CD8 alpha antibody [EPR21769] (ab217344) is a rabbit monoclonal antibody detecting CD8 alpha in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr . Suitable for Mouse . - Biophysical QC for unrivalled batch-batch consistency - Over 160 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21769,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:IHC-Fr, Flow Cyt, IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CD8 alpha antibody [EPR21769] (ab217344) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, IHC-Fr IHC-P, IP and WB.
Anti-CD8 alpha antibody [EPR21769] (ab217344) was first used in a scientific publication in 2019 and has been cited over 164 times in peer reviewed journals. It's performance in IHC and Immunofluorescence in mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD8 alpha antibody [EPR21769] (ab217344) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD8 alpha antibody [EPR21769] (ab217344) has 4 independent reviews from customers.
Anti-CD8 alpha antibody [EPR21769] (ab217344) specifically detects CD8 alpha (UniProt ID: P01731; Molecular weight: 25kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR21769 -
ab230156
Antibody clone EPR21769 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, FITC, APC, Alexa Fluor
594, Alexa Fluor
555, PE (
ab237364
ab237365
ab237367
ab237368
ab277939
ab280863
ab306536
CD8 alpha is involved in diseases by aiding cytotoxic T cells in targeting and eliminating infected or malignant cells. CD8 T cell dysfunction is associated with several diseases including Autoimmune disease, cancer and infectious diseases.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
Biological function summary
The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.
Pathways
CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.
CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.