Abcam, ab263333, Human RPS27L knockout HeLa cell lysate

Size: 1Kit
RPS27L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon2 and 7 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon2 and 7 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RPS27L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RPS27L also known as Ribosomal Protein S27-like or Metallopanstimulin-1 (MPS-1) is a component of the ribosome with a molecular weight of approximately 9.5 kDa. It is expressed across various tissues particularly in liver and prostate. Mechanically RPS27L functions as part of the ribosomal machinery where it plays a role in the assembly and stability of ribosomal constituents necessary for protein synthesis.
Biological function summary
This protein contributes to the regulation of the cellular response to DNA damage and is involved in the control of cell cycle progression. It is part of the small subunit of the ribosome where it associates with other ribosomal proteins to form a functional ribosomal unit. Its activity emphasizes its importance in maintaining genomic stability and proper cellular functions.
Pathways
RPS27L plays a substantial role in the DNA damage response and the p53 signaling pathway. It interacts with the p53 protein an important tumor suppressor regulating p53 levels and activity following genotoxic stress. The interplay between RPS27L and p53 emphasizes its involvement in pathways that oversee damage repair mechanisms particularly by influencing p53-mediated transcriptional regulation and apoptosis.
RPS27L has notable associations with cancer and ribosomopathies. Aberrant function or expression of RPS27L can contribute to tumorigenesis often through its interplay with the p53 pathway. Additionally it connects with proteins such as MDM2 a critical regulator of p53 in the context of oncogenic disorders highlighting its potential as a therapeutic target in related malignancies.