Thermo Fisher, L101503, SuperScript™ Direct cDNA Labeling Module, no purification

SuperScript™ Direct cDNA Labeling Module, no purification The SuperScript™ Direct cDNA Labeling System is a microarray labeling kit that combines the performance of SuperScript™ III Reverse Transcriptase (RT) with the simplicity of direct labeling to yield highly fluorescent labeled cDNA that accurately represents your initial mRNA sample. In this method (Figure 1), anchored oligo(dT) anneals to the mRNA template. SuperScript™ III RT extends from the priming site, incorporating fluorescent dNTPs directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using purification columns before hybridization to a microarray.
The SuperScript™ Direct cDNA Labeling System uses 800 units of SuperScript™ III RT to generate:
• High cDNA yields without an additional enzyme “spike” (Figure 2)
• Strong signal intensities (Figure 3)
• A fast and simple protocol that minimizes “hands on” time and provides superior reproducibilityDetection Method: Fluorescence
Final Product Type: cDNA (Labeled)
Includes Label or Dye: No
Labeling Method: Direct Labeling
Labeling Target: cDNA
Label or Dye: Alexa Fluor™ 555, Alexa Fluor™ 647, Cy™3, Cy™5
Product Line: SuperScript™
Product Type: cDNA Labeling Module
Quantity: 30 Reactions
Sample Type: RNA
Unit Size: Each