Abcam, ab105472, Anti-S100A9 antibody [2B10]

Size: 100µg
Anti-S100A9 antibody [2B10] (ab105472) is a rat monoclonal antibody detecting S100A9 in IHC-P, ICC/IF . Suitable for Human, Mouse . - Over 20 publications
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:2B10,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Mouse S100a9.P31725,
Specificity:Specific for mouse S100-A9 protein that forms a heterodimer with S100-A8 and constitutes 45% of the neutrophil cytosolic protein. Stains neutrophils strongly and monocytes weakly.

Product details:
What is this antibody validated in?
Anti-S100A9 antibody [2B10] (ab105472) is a rat monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
Trusted by the scientific community
Anti-S100A9 [2B10] (ab105472) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
S100A9 also known as calgranulin B or MRP-14 is a calcium-binding protein with a molecular weight of approximately 13 kDa. It belongs to the S100 family and forms part of a heterodimeric complex with S100A8. S100A9 is largely found in myeloid cells such as neutrophils and monocytes. It plays a significant role in the cytosol and can be secreted to the extracellular space under specific stress conditions.
Biological function summary
S100A9 impacts inflammatory responses and immune regulation. It partners with S100A8 to form the calprotectin complex which acts as a strong pro-inflammatory mediator. This complex binds to receptors like RAGE and TLR4 initiating signaling pathways that promote inflammation. S100A9 also participates in leukocyte recruitment and has antimicrobial properties blocking the growth of bacteria and fungi by chelating essential metal ions.
Pathways
Several are influenced by S100A9 including the NF-kB and MAPK signaling pathways. Through its interaction with RAGE and TLR4 receptors S100A9 influences the activation of these pathways which are important in the regulation of inflammatory and immune responses. Other proteins involved in these pathways include MyD88 for TLR4 signaling and various kinases for MAPK signaling positioning S100A9 as an important regulator within the inflammatory networks.
S100A9 links to various inflammatory and autoimmune conditions such as rheumatoid arthritis and inflammatory bowel disease. Increased levels of S100A9 have been observed in these conditions where it augments the inflammatory response. In rheumatoid arthritis for example S100A9 upregulation correlates with increased joint inflammation and damage. The protein’s interaction with S100A8 in these diseases highlights its contribution to the pathogenesis and progression of inflammation-related conditions.