Abcam, ab22674, Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231]

Size: 50µL
Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674) is a mouse monoclonal antibody detecting Proteasome 20S alpha 1+2+3+5+6+7 in Flow Cytometry, ICC/IF . Suitable for Human . - Over 20 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:MCP231,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Full Length Protein corresponding to Human PSMA1. The exact immunogen used to generate this antibody is proprietary information.P25786,
Epitope:Reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to alpha type subunits, although not necessarily identical in all. In general this motif is phylogenetically preserved.

Product details:
What is this antibody validated in?
Anti-Proteasome 20S alpha 1+2+3+5+6+7 antibody [MCP231] (ab22674) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Trusted by the scientific community
Anti-Proteasome 20S alpha 1+2+3+5+6+7 [MCP231] (ab22674) was first used in a scientific publication in 2005 and has been cited over 20 times in peer-reviewed journals.
Reviewed by scientists
Anti-Proteasome 20S alpha 1+2+3+5+6+7 [MCP231] (ab22674) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Proteasome 20S alpha subunits (also known as proteasome marker proscrate) are parts of the core structure of the 20S proteasome. This target features the six alpha subunits: alpha 1 alpha 2 alpha 3 alpha 5 alpha 6 and alpha 7. These alpha subunits collectively form the gate of the proteolytic chamber regulating substrate entry. The mass of the entire 20S proteasome complex is around 700 kDa. This target is ubiquitously expressed in eukaryotic cells playing a critical role in protein degradation.
Biological function summary
The 20S alpha subunit functions as part of the larger 26S proteasome complex which includes other components such as 19S regulatory particles. It facilitates protein turnover by degrading misfolded damaged or unneeded proteins into peptides. The 20S proteasome subunits' activity is essential in maintaining cellular homeostasis and controlling the concentration of specific proteins. They contribute to processes like cell cycle regulation and antigen presentation.
Pathways
The 20S proteasome involves itself directly in the ubiquitin-proteasome pathway a fundamental pathway for protein degradation. It is an important player in the immune response pathway aiding in processing peptides for major histocompatibility complex (MHC) class I antigen presentation. This function connects the 20S proteasome to other proteins such as ubiquitin and molecules involved in antigen processing.
Dysregulation of 20S proteasome activity associates with conditions such as cancer and neurodegenerative diseases. In cancer increased proteasome activity supports rapid cell division by degrading cell cycle inhibitors. Furthermore research links impaired proteasome function in neurodegenerative disorders such as Alzheimer's disease where inefficient protein degradation leads to the accumulation of toxic protein aggregates. The target connects to proteins like amyloid precursor protein in Alzheimer's emphasizing the critical role of proteasome subunits in disease pathogenesis.