Abcam, ab279707, Anti-Cbx6 antibody [EPR23828-108] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CBX6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23828-108,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab279707 is the carrier-free version of
ab259848
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cbx6 also known as Chromobox Protein Homolog 6 is a component of the Polycomb Group (PcG) proteins with a core function in transcriptional repression. It has a molecular mass of approximately 51 kDa. The primary expression of Cbx6 occurs in stem and progenitor cells but it is also present in various tissues during embryogenesis. The protein contains a chromodomain that facilitates its interaction with histones and contributes to chromatin structure alteration impacting gene expression regulation.
Biological function summary
Cbx6 plays a significant role in maintaining cellular pluripotency and regulating differentiation processes. As part of the Polycomb Repressive Complex 1 (PRC1) Cbx6 participates in modifying chromatin to silence target genes. This complex works synergistically with other PcG proteins to ensure correct temporal and spatial expression of developmentally important genes. The association with histone H3 lysine 27 trimethylation (H3K27me3) is fundamental to its repressive function.
Pathways
Cbx6 functions integrally within the epigenetic regulation pathway and impacts the Wnt signaling pathway. By interacting with other PRC1 members Cbx6 facilitates transcriptional silencing required for the maintenance of stem cell identity. Its activities are closely linked to the modulation of canonical Wnt signaling and in doing so it affects proteins such as β-catenin as well as interacting with other PcG components like Cbx7 and Bmi1.
Cbx6 shows associations with various cancers and developmental disorders. Its dysregulation is observed in malignancies like colorectal cancer where abnormal Cbx6 expression affects the Wnt signaling pathway leading to enhanced proliferation. Furthermore disorders in embryonic development can arise from improper Cbx6 function in Polycomb-mediated gene silencing. Within the context of these conditions Cbx6 works alongside other regulators such as EZH2 in the PRC2 complex highlighting its multifaceted role in disease states.