Agilent, 103020-100, Seahorse XF Glycolysis Stress Test Kit

Seahorse XF Glycolysis Stress Test Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of glucose, oligomycin, and 2-deoxy-D-glucose. For use with Seahorse XFe/XF96 and XF/XF24 analyzers.

Specifications:
Assay Wells: 6 x 96-well or 24-well assays
Assays per Kit: 6
For Use With: Seahorse XF96 AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 Analyzer
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Store at room temperature

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The Seahorse XF Glycolysis Stress Test Kit enables real-time quantification of glycolytic function in live cells by measuring extracellular acidification rates (ECAR) using Agilent Seahorse XF Analyzers. This kit provides a validated, optimized assay workflow to assess key parameters including glycolysis, glycolytic capacity, and glycolytic reserve. By sequentially injecting glucose, oligomycin, and 2-deoxy-glucose during the assay, researchers can accurately profile metabolic responses and pathway flux under various experimental conditions. Typical usage scenarios include the characterization of metabolic phenotypes in oncology, immunology, metabolic disease studies, and drug development or screening. The kit is especially valuable for investigating metabolic reprogramming and the impact of genetic or environmental perturbations on cellular energy metabolism. It supports a rapid, high-throughput analysis format that minimizes sample handling and maximizes data quality. For optimal performance, it is designed for use with Agilent Seahorse XF24, XF96, XFe24, and XFe96 Analyzers, as well as related cell culture and assay products in the Agilent Seahorse portfolio. This kit streamlines assay setup and data interpretation, offering a robust solution for researchers studying cell metabolism and bioenergetic function across a broad range of mammalian cell types.