Biolegend, 106003, Biotin anti-mouse CD123 Antibody, 50μg

CD123 is a 70 kD α chain subunit of the IL-3 receptor (IL-3R α). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common β chain (CDw131) or the IL-3R β chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R β subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CTLL cells transfected with the mouse IL-3Rα chain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106004) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.
Application References(PubMed link indicates BioLegend citation): Ichihara, et al. 1995. EMBO J. 14:939. (FC) Mueller DL, et al. 1994. J. Immunol. 153:3014. (FC) Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC)
Product Citations: Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
RRID: AB_2124404 (BioLegend Cat. No. 106003)
Structure: Ig superfamily, associates with CDw131 (common β chain) or IL-3 receptor β chain, 70 kD
Distribution: Hematopoietic progenitors, basophils, mast cells, megakaryocytes
Function: Proliferation, differentiation of hematopoietic cells
Ligand/Receptor: IL-3
Cell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Miyajima A, et al. 1993. Blood 82:1960. 3. Itoh N, et al. 1990. Science 247:324.
Gene ID: 16188
UniProt: View information about CD123 on UniProt.org
Clone: 5B11
Regulatory Status: RUO
Other Names: IL-3 Receptor α chain, IL-3Rα
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.