Biolegend, 123521, APC anti-mouse CD1d (CD1.1, Ly-38) Antibody, 25μg

CD1d, also known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein that is structurally similar to MHC class I and is non-covalently associated with β2-microglobulin. In humans, the CD1 family consists of group I (CD1a, CD1b, and CD1c), group II (CD1d), and group III (CD1e) proteins, but CD1d is the only CD1 molecule found in mice. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes. CD1d plays a role in presenting non-peptide glycolipid antigens to CD1d-restricted T cells, and PKCδ has been identified as a critical regulator of CD1d-mediated antigen presentation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD1.1 cDNA-transfected RMA-S mouse T lymphoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistical staining, and blocking function3. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.
Application References(PubMed link indicates BioLegend citation): Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block) Brozovic S, et al. 2004. Nat. Med. 10:535. Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block) Jiang J, et al. 2012. PLoS One. 7:47487. PubMed
Product Citations: Crouch MJ, et al. 2019. J Leukoc Biol. 241:106. PubMed Meednu N, et al. 2022. Cell Rep. 110766:39. PubMed Lepore F et al. 2018. Frontiers in immunology. 2.284722222 . PubMed Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
RRID: AB_2715919 (BioLegend Cat. No. 123521) AB_2715920 (BioLegend Cat. No. 123522)
Structure: 48 kD glycoprotein, Ig superfamily
Distribution: leukocytes, dendritic cells, epithelial cells, thymocytes
Function: Antigen presentation
Ligand/Receptor: similar to MHC class I, associate with ß-microglobulin
Cell Type: Dendritic cells, Epithelial cells, Leukocytes, Thymocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Brudin N, et al. 1998. J. Immunol. 161:3271. 2. Amano M, et al. 1998. J. Immunol. 161:1710. 3. Brossay L, et al. 1997. J. Immunol. 159:1216. 4. Dougan SK, et al. 2007. Curr. Top. Microbiol. Immunol. 314:113. 5. Brutkiewicz RR, et al. 2007. Eur. J. Immunol. 37:2390.
Gene ID: 12479
UniProt: View information about CD1d on UniProt.org
Clone: 1B1
Regulatory Status: RUO
Other Names: CD1, CD1.1, Ly-38
Isotype: Rat IgG2b, κ