Biolegend, 141612, PE/Dazzle™ 594 anti-mouse H-2K, 100μg

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo .
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SIINFEKL pulsed RMA-S cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.
Application References(PubMed link indicates BioLegend citation): Mareeva T, et al. 2010. J. Immunol. Methods 353:78. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF) Porgador A, et al. 1997. Immunity 6:715. (IF, IHC) Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.
RRID: AB_2750521 (BioLegend Cat. No. 141611) AB_2750522 (BioLegend Cat. No. 141612)
Structure: OVA peptide257-264 and H-2Kb complex
Distribution: SIINFEKL bond H-2Kb bearing cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. 2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053. 3. Deng Y, et al. 1998. J. Immunol. 161:1677.
Gene ID: 14972
UniProt: View information about H-2Kb bound to SIINFEKL on UniProt.org
Clone: 25-D1.16
Regulatory Status: RUO
Other Names: MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb
Isotype: Mouse IgG1, κ