Biolegend, 142421, Brilliant Violet 421™ anti-mouse CD169 (Siglec-1) Antibody, 50μg

CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains). CD169 binds to sialic acids, which can be found on PSGL-1, CD43, CD206, and CD227. By its affinity to α2, 3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells. CD169 was initially identified as a sialic acid-dependent sheep erythrocyte receptor (SER) on resident bone marrow cells of mice. It has been identified as highly expressed on resident bone marrow macrophages which plays an important role in retention of stem cells in mesenchymal stem cell niche. It is also found on some specific subsets of tissue macrophages in spleen, lymph nodes, bone marrow, liver, colon, lungs, and cancer cells. Evidence suggest that CD169-positive macrophages serve as lymph node-resident APCs to dominate early activation of tumor antigen-specific CD8 + T cells and invariant NK cell.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Purified Native Sialoadhesin from spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-F - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections1, and spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F) Chtanova T, et al. 2008. Immunity 29:487. Klass M, et al. 2012. J. Immunol. 189:2414. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Suzuki Y, et al. 2022. Mol Ther Nucleic Acids. 30:226. PubMed Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed Ma X, et al. 2020. Immunity. 53:1315. PubMed Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
RRID: AB_2734202 (BioLegend Cat. No. 142421)
Structure: Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution: Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.
Function: Adhesion.
Ligand/Receptor: PSGL-1, CD43, CD206 and CD227.
Cell Type: Macrophages
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References: 1. Chow A, et al. 2011. J. Exp. Med. 208:261. 2. Asano K, et al. 2011. Immunity 34:85. 3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057. 4. Varki A, et al. 2009. Glycoconj. J. 26:231. 5. Rempel H, et al. 2008. PLoS One 3:e1967. 6. Crocker PR, et al. 2001. Trends Immunol. 22:337. 7. Hartnell A, et al. 2001. Blood 97:288. 8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.
Gene ID: 20612
UniProt: View information about CD169 on UniProt.org
Clone: 3D6.112
Regulatory Status: RUO
Other Names: Sialic acid binding Ig-like lectin 1 (Siglec-1), Sialoadhesin (Sn)
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.