Biolegend, 149204, PE anti-mouse ENPP1 Antibody, 100μg

ENPP1, also known as PC-1 or Ly-41, is a 115 kD type II transmembrane enzyme that is located on the cell's surface as a homodimer. ENPP1 hydrolyses ATP and other nucleoside 5' triphosphates to produce pyrophosphate which is an inhibitor of calcification and bone formation. It is involved in nucleotide recycling, cell adhesion, and regulates the signaling of insulin and purinergic receptors. ENPP1 is expressed by plasma cells, subsets of T and B1 cells, chondrocytes, capillary endothelium of the brain, renal distal tubules, and hepatocytes.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine T cell lymphoma EL4.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This clone recognizes both alleles of ENPP1.Additional reported applications (for the relevant formats) include: Immunoprecipitation.2
Application References(PubMed link indicates BioLegend citation): Wang H, et al. 2012. Proc Natl Acad Sci USA 109:20077. (FC)Abbasi S, et al. 2011. Hybridoma 30:11. (FC, IP)Waterfield JD and Fairhurst M. 1991. Immunology 72:226. (FC)Takei F. 1984. J. Immunol. 4:1951. (FC, IP)
Product Citations: Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
RRID: AB_2564619 (BioLegend Cat. No. 149203) AB_2564620 (BioLegend Cat. No. 149204)
Structure: Type II transmembrane protein, 115 kD, and forms homodimers.
Distribution: Plasma cells, a subset of T cells, a subset of B1 cells, chondrocytes, capillary endothelium of the brain, renal distal tubules, and hepatocytes.
Function: Generate pyrophosphate, recycle nucleotides, regulate cell adhesion, and to regulate signaling of insulin and purinergenic receptors.
Cell Type: Plasma cells, T cells, B cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins
Antigen References: 1. Lau WM, et al. 2013. PLoS One. 8:e66752.2. Kato K, et al. 2012. Proc. Natl. Acad. Sci. USA 109:16876.3. Nam HK, et al. 2011. J Biol. Chem. 286:39059.4. Zhou HH, et al. 2009. Eur. J. Pharmacol. 616:346.
Gene ID: 18605
UniProt: View information about ENPP1 on UniProt.org
Clone: YE1/19.1
Regulatory Status: RUO
Other Names: Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), Ly-41, PC-1, NPP1, Plasma Cell Alloantigen 1 (PC1)
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.