Biolegend, 304821, PE/Cyanine7 anti-human CD62L Antibody, 25tests

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
25tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block) Jutila M, et al. 2002. J. Immunol. 169:1768. (WB) Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB) Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Product Citations: Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed Ye L, et al. 2022. Cell Metab. 34:595. PubMed Das RK, et al. 2019. Cancer Discov. 9:492. PubMed Kim C, et al. 2018. Cell Rep. 25:2148. PubMed Konadu K, et al. 2015. J Infect Dis. 211:1712. PubMed Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed Giamarellos-Bourboulis EJ, et al. 2020. Cell. 183(2):315-323.e9. PubMed Toews K, et al. 2020. Mol Carcinog. 724:59. PubMed Moorlag SJCFM, et al. 2020. Cell Rep. 33:108387. PubMed Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed Theobald SJ, et al. 2022. EMBO Mol Med. 14:e15888. PubMed Hu N, et al. 2014. PLoS One. 9:99671. PubMed Singh N, et al. 2016. Sci Transl Med. 8: 320ra3. PubMed Veerapathran A, et al. 2011. Blood. 118:5671. PubMed Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed Liao HQ, et al. 2021. Am J Reprod Immunol. 86:e13406. PubMed Crompton J, et al. 2015. Cancer Res. 75:296. PubMed Zou F, et al. 2019. Nat Commun. 10:4109. PubMed
RRID: AB_830800 (BioLegend Cat. No. 304821) AB_830801 (BioLegend Cat. No. 304822)
Structure: Selectin, single chain glycoprotein, 74-95 kD
Distribution: Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
Function: Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor: CD34, GlyCAM, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. Kishimoto T, et al. 1991. Blood 78:805.
Gene ID: 6402
UniProt: View information about CD62L on UniProt.org
Clone: DREG-56
Regulatory Status: RUO
Workshop: V S056
Other Names: L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype: Mouse IgG1, κ