Biolegend, 320012, Alexa Fluor® 488 anti-mouse/rat/human FOXP3 Antibody, 100tests

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4 + /CD25 - cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.
100tests
Verified Reactivity: Human, Mouse, Rat
Reported Reactivity: Cynomolgus, Rhesus, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full-length FOXP3 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
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Product Citations: Xie L, et al. 2014. J Immunol. 192:6009. PubMed Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed Chaurio RA, et al. 2022. Immunity. 55:115. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Yu J, et al. 2022. Nat Commun. 13:7903. PubMed Valencia X, et al. 2007. J Immunol . 178:2579. PubMed Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed Li Q, et al. 2022. Cell Rep. 40:111308. PubMed Zhu P, et al. 2012. J Cereb Blood Flow Metab. 0.482638889. PubMed Berg S 2006. J Immunol. 176:2059. PubMed Gkountidi AO, et al. 2021. Cancer Immunol Res. 9:748. PubMed Xiong Y, et al. 2012. J Biol Chem. 287:34372. PubMed Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed Renga G, et al. 2020. Life Sci Alliance. 3:. PubMed Meng KP, et al. 2020. J Exp Med. 217:00:00. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed Perkins T, et al. 2017. J Allergy Clin Immunol Pract. 10.1016/j.jaip.2017.01.028. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed An J, et al. 2022. iScience. 25:103570. PubMed PRADO-GARCIA H, et al. 2015. Anticancer Res. 35:1529. PubMed Shi R, et al. 2022. Theranostics. 12:875. PubMed Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed Becher J, et al. 2018. Dev Cell. 47:592. PubMed Cimbro R, et al. 2014. Proc Natl Acad Sci U S A. 6:81. PubMed Liu B, et al. 2022. Mol Med Rep. 26:. PubMed Murakami R, et al. 2013. PLoS One. 8:73270. PubMed Oida T, Weiner H 2010. PLoS One. 5:e15523. PubMed Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed Martínez–Fábregas J, et al. 2019. Elife. 8:e49314. PubMed Zhu D, et al. 2017. Stem Cell Res Ther. 0.511805556. PubMed Luck H, et al. 2019. Nat Commun. 10:3650. PubMed Machhi J, et al. 2021. J Neuroinflammation. 18:272. PubMed Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed Nakazawa Y, et al. 2021. Elife. 10:. PubMed Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed Golovina T, et al. 2008. J Immunol. 181:2855. PubMed Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID: AB_439747 (BioLegend Cat. No. 320011) AB_439748 (BioLegend Cat. No. 320012)
Structure: Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution: Nuclear; expressed in T regulatory cells
Function: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction: Interacts with DNA
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Hori S, et al. 2003. Science 299:1057.
Regulation: FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Gene ID: 2037131738250943
UniProt: View information about FOXP3 on UniProt.org
Clone: 150D
Regulatory Status: RUO
Other Names: Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype: Mouse IgG1, κ
Q: Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?
A: It is not recommended. It is best to use PBMCs for this testing.
Q: Can FOXP3 be costained with cytokines?
A: The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.