Biolegend, 333014, PerCP/Cyanine5.5 anti-human CD85k (ILT3) Antibody, 100tests

CD85k, known as Immunoglobulin-like transcript (ILT) 3, is an inhibitory receptor expressed by dendritic cells and a subset of white blood cells called monocytes.  ILT3, also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses. Expression of ILT3 is up-regulated on tolerogenic dendritic cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: ILT3 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
RRID: AB_2565559 (BioLegend Cat. No. 333014)
Structure: Immunoglobulin superfamily
Distribution: Monocytes, Macrophages, and Dendritic Cells
Cell Type: Dendritic cells, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Cella M, et al. 1997. J Exp Med. 185:1743-51. Penna G, et al. 2005. Blood. 5:2044.
Gene ID: 11006
UniProt: View information about CD85k on UniProt.org
Clone: ZM4.1
Regulatory Status: RUO
Other Names: Immunoglobulin-like transcript (ILT) 3, CD85k, Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4), Leukocyte Immunoglobulin-like Receptor 5 (LIR-5)
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.