Biolegend, 385607, PE anti-human CD25 Antibody, 25tests

CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T-Cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD25 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S20019C can completely block binding of clone BC96 and clone TÜ69 on target cells and can be blocked by BC96 and by TÜ69.Clone S20019C does not block the binding of clone S20019D and clone M-A251 on target cells.Clone S20019C can block IL-2 binding to receptor.Clone S20019C shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
RRID: AB_3068169 (BioLegend Cat. No. 385607) AB_3068169 (BioLegend Cat. No. 385608)
Structure: 55 kD transmembrane protein
Distribution: Activated T-Cells, T regulatory Cells, monocytes
Function: IL-2 receptor
Interaction: CD122, CD132
Ligand/Receptor: IL-2
Cell Type: Lymphocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Waldmann T. 1991. J Biol. Chem. 266:2681-4. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: S20019C
Regulatory Status: RUO
Other Names: interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.