Biolegend, 386104, Ultra-LEAF™ Purified anti-human CD66c Antibody, 1mg

Carcinoembryonic antigen (CEA) Cell Adhesion Molecule 6 (CEACAM6) belongs to the (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. As a cell adhesion molecule, CEACAM6 mediates homotypic binding with other CEA family members and heterotypic binding with integrin receptors. CEACAM6 is expressed on epithelial surfaces and in the myeloid lineage. Despite lacking transmembrane and intracellular domains, GPI-anchored proteins, including CEACAM family members, are able to influence transmembrane signaling via tyrosine kinases. CEACAM6 modulates c-Src tyrosine kinase activity. Overexpressed in more than 50% of all human adenocarcinomas, CEACAM6 promotes aberrant cell differentiation, anti-apoptosis, cell growth and resistance to therapeutic agents, cell invasion and metastasis.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein, aa 35-320
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: ICC - Quality tested FC, Blocking, Activation - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2941566 (BioLegend Cat. No. 386103) AB_2941566 (BioLegend Cat. No. 386104)
Structure: Glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins Immunoglobulin superfamily
Distribution: Apical Cell Membrane
Function: The extracellular N-terminus Ig-like V-type domain is necessary for homophilic and heterophilic intercellular adhesion.
Interaction: Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8
Cell Type: Epithelial cells, Granulocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Duxbury MS, et al. 2004. Oncogene. 23:465-73. Oikawa S, et al. 1991. J Biol Chem. 266:7995-8001. Noworolska A, et al. 1986. Cancer Detect Prev. 9:365-71. Wu S, et al. 2021. Transl Oncol. 14:101057
Gene ID: 4680
UniProt: View information about CD66c on UniProt.org
Clone: W20030E
Regulatory Status: RUO
Other Names: Carcinoembryonic antigen-related cell adhesion molecule 6
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.