Biolegend, 400289, Ultra-LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl Antibody, 50mg

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.
50mg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality testedICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Product Citations: Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed van der Sluis RM, et al. 2022. EMBO J. 41:e109622. PubMed Montes de Oca M, et al. 2016. Cell Rep. 17:399-412. PubMed Sugimoto C, et al. 2022. Elife. 11:. PubMed Abe S, et al. 2020. JCI Insight. 5:00. PubMed Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed Lee Y, et al. 2022. Front Immunol. 12:780210. PubMed Langer V, et al. 2019. J Clin Invest. 129:4691. PubMed Arakawa A, et al. 2021. J Immunol. 207:2235. PubMed Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed Kaur A, et al. 2016. Nature. 532: 250-254. PubMed Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed
RRID: AB_11149166 (BioLegend Cat. No. 400263) AB_11148947 (BioLegend Cat. No. 400264) AB_11148947 (BioLegend Cat. No. 400281) AB_11148947 (BioLegend Cat. No. 400282) AB_11148947 (BioLegend Cat. No. 400289) AB_11148947 (BioLegend Cat. No. 400290)
Gene ID: NA
Clone: MOPC-173
Regulatory Status: RUO
Isotype: Mouse IgG2a, κ
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.