Biolegend, 400968, Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl Antibody, 100mg

This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100mg
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Trinitrophenol + KLH
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC, ICFC - Quality tested ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717. Yu R, et al. 2006. Obesity 14:1353. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed
Product Citations: Arnholdt C, et al. 2022. Cells. 11:. PubMed Katsuumi G, et al. 2024. Nat Aging. 926:4. PubMed Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed Tang Y, et al. 2022. Dev Cell. 57:480. PubMed Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed Gounder AP, et al. 2018. PLoS Pathog. 14:e1007001. PubMed Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed Gyorke CE, et al. 2020. J Immunol. 205:3037. PubMed Deng KM, et al. 2021. Cells. 10:. PubMed Olafsson EB, et al. 2019. J Cell Sci. 132. PubMed Ross EC, et al. 2021. Cell Mol Life Sci. 78:5197. PubMed Ji DX, et al. 2019. Nat Microbiol. 1.644444444. PubMed Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed Zhou HM, et al. 2020. Exp Dermatol. 29:102. PubMed Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed He C, et al. 2022. JCI Insight. 7:. PubMed
RRID: AB_11203529 (BioLegend Cat. No. 400969) AB_11203529 (BioLegend Cat. No. 400940) AB_11203529 (BioLegend Cat. No. 400959) AB_11203529 (BioLegend Cat. No. 400960) AB_11203529 (BioLegend Cat. No. 400967) AB_11203529 (BioLegend Cat. No. 400968)
Gene ID: NA
Clone: HTK888
Regulatory Status: RUO
Isotype: Armenian Hamster IgG
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.